Journal: mBio
Article Title: Identification of NECTIN1 as a novel restriction factor for flavivirus infection
doi: 10.1128/mbio.02708-24
Figure Lengend Snippet: NECTIN1 competes with CD46 for binding to BVDV E2. ( A ) Schematic of the interaction between NECTIN1, BVDV-E2, and CD46. ( B ) Confocal identification of BVDV-E2 and CD46, BVDV-E2 and NECTIN1, and NECTIN1 and CD46. MYC-vector, HA-vector, and FLAG-vector were used as controls. The fluorescence signal in the pink line area was analyzed by the intensity peak diagram. Scale bar, 5 µm. ( C ) Western blot identification of the expression levels of NECTIN1 and CD46 on the plasma membrane. ( D ) Western blot analysis of the interactions between BVDV-E2 and CD46, between BVDV-E2 and NECTIN1, and between CD46 and NECTIN1. MYC-vector, HA-vector, and FLAG-vector were used as controls. ( E ) The schematic diagram of the four domains of BVDV E2. ( F ) Western blot analysis of the interactions between the four domains of BVDV-E2 and NECTIN1. ( G ) Western blot analysis of the interaction between CD46, NECTIN1, and BVDV-E2. HA-vector was used as a control. ( H ) SDS-PAGE analysis of the expression of NECTIN1, CD46, and BVDV E2. ( I ) SPR identification of the binding affinity of BVDV E2 for CD46. ( J ) SPR identification of the binding affinity of BVDV E2 for NECTIN1.
Article Snippet: The bovine CD46 protein was purchased from MedChemExpress (MCE).
Techniques: Binding Assay, Plasmid Preparation, Fluorescence, Western Blot, Expressing, Clinical Proteomics, Membrane, Control, SDS Page